Heart, Lung and Circulation
Volume 16, Issue 2 , Pages 85-92, April 2007

Early-phase Endotoxic Shock-induced Myocardial Injury Increases iNOS and Selectin Expression in Macaque Primate

  • Guo Qing Yin, MD, PhD

      Affiliations

    • College of Veterinary Medicine, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, PR China
    • The Second Hospital of Nanjing, affiliated with Medical College, Southeast University, 1-1 Zhongfu Road, Nanjing, Jiangsu 210003, PR China
    • Corresponding Author InformationCorresponding author at: 1-1 Zhongfu Road, Nanjing, Jiangsu 210003, PR China. Tel.: +86 25 83465622; fax: +86 25 83466026.
    • ,
  • Kai He Du

      Affiliations

    • Department of Biology of Nanjing Normal University, 122 Ninghai Road, Nanjing, Jiangsu 210024, PR China
    • ,
  • Fei Rong Gu

      Affiliations

    • College of Veterinary Medicine, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, PR China
    • ,
  • Zhi Xun Fang, MD

      Affiliations

    • The Second Hospital of Nanjing, affiliated with Medical College, Southeast University, 1-1 Zhongfu Road, Nanjing, Jiangsu 210003, PR China
    • ,
  • Jia Qi Tang, MD

      Affiliations

    • College of Veterinary Medicine, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, PR China
    • ,
  • Bei Zhong, MD

      Affiliations

    • The Second Hospital of Nanjing, affiliated with Medical College, Southeast University, 1-1 Zhongfu Road, Nanjing, Jiangsu 210003, PR China
    • ,
  • Xiao Yun Zhu, MD

      Affiliations

    • The Second Hospital of Nanjing, affiliated with Medical College, Southeast University, 1-1 Zhongfu Road, Nanjing, Jiangsu 210003, PR China
    • ,
  • Ying Wei Wu

      Affiliations

    • The Second Hospital of Nanjing, affiliated with Medical College, Southeast University, 1-1 Zhongfu Road, Nanjing, Jiangsu 210003, PR China
    • ,
  • Cheng Ping Lu, PhD

      Affiliations

    • College of Veterinary Medicine, Nanjing Agricultural University, 6 Tongwei Road, Nanjing, Jiangsu 210095, PR China
    • Corresponding Author InformationCorresponding author. Tel.: +86 25 84396517; fax: +86 25 84396517.

Received 10 July 2006; received in revised form 6 October 2006; accepted 11 October 2006.

Background

Our previous study has established a macaque model with early-phase endotoxic shock. The present study further investigated myocardial and blood vessel injury in Macaques by examining the subsequent expression of ACP, selectins, iNOS, and cTnI in response to LPS treatment.

Methods

In an experiment with anaesthetised, instrumental macaques, eleven animals were randomised into: an En group (n=6), receiving a dose of 2.8mgkg−1 lipopolysaccharides (LPS) by i.v.; and a Co group (n=5), injected with normal saline of 1mlkg−1. Cytochemistry of acid phosphatase (ACPase) in heart was performed by electron microscope at 120min following endotoxin injection. Three immunochemical stains, namely, L-selectin, P-selectin and iNOS protein in heart, were studied. In addition, cardiac troponin I (cTnI), L-selectin and P-selectin in plasma were detected.

Results

In the early phase of endotoxic shock, LPS caused myocardial lysosome damage. The data of immunochemical staining showed the thrombus formation in vessels and the increase of iNOS, L-Selectin and P-Selectin expression in heart, but LPS challenge did not change L-selectin, P-selectin and cTnI in plasma.

Conclusion

The increase of iNOS, L-selectin and P-selectin protein expression following endotoxin administration may have caused vessel injury and myocardial damage in macaques.

Keywords: Entotoxin, Acid phosphatase, Selectin, NO synthase, Cardiac muscle, Macaque

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PII: S1443-9506(06)00235-6

doi:10.1016/j.hlc.2006.10.006

Heart, Lung and Circulation
Volume 16, Issue 2 , Pages 85-92, April 2007

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