Heart, Lung and Circulation
Volume 17, Issue 5 , Pages 395-403 , October 2008

Mesenchymal Stem Cells: Isolation, Characterisation and In Vivo Fluorescent Dye Tracking

  • Christopher Weir, PhD

      Affiliations

    • Cardiac Technology Centre & Kolling Institute, Sydney, Australia
    • Northern Blood Research Centre and Department of Haematology, Sydney, Australia
    • University of Sydney at Royal North Shore Hospital, Sydney, Australia
    • ,
  • Marie-Christine Morel-Kopp, PhD

      Affiliations

    • Northern Blood Research Centre and Department of Haematology, Sydney, Australia
    • University of Sydney at Royal North Shore Hospital, Sydney, Australia
    • ,
  • Anthony Gill, FRCPA

      Affiliations

    • Anatomical Pathology Department, Sydney, Australia
    • University of Sydney at Royal North Shore Hospital, Sydney, Australia
    • ,
  • Kellie Tinworth, MSc

      Affiliations

    • Cardiac Technology Centre & Kolling Institute, Sydney, Australia
    • University of Sydney at Royal North Shore Hospital, Sydney, Australia
    • ,
  • Leigh Ladd, PhD

      Affiliations

    • Cardiac Technology Centre & Kolling Institute, Sydney, Australia
    • University of Sydney at Royal North Shore Hospital, Sydney, Australia
    • ,
  • Stephen N. Hunyor, MD

      Affiliations

    • Cardiac Technology Centre & Kolling Institute, Sydney, Australia
    • University of Sydney at Royal North Shore Hospital, Sydney, Australia
    • Corresponding Author InformationCorresponding author at: Cardiac Technology Centre, Department of Cardiology, Royal North Shore Hospital, St Leonards, Sydney, NSW 2065, Australia. Tel.: +61 2 9926 8679; fax: +61 2 9901 4097.
    • ,
  • Christopher Ward, PhD

      Affiliations

    • Northern Blood Research Centre and Department of Haematology, Sydney, Australia
    • University of Sydney at Royal North Shore Hospital, Sydney, Australia

Received 13 December 2007 ,Revised 15 January 2008 ,Accepted 20 January 2008.

References 

  1. Li WJ, Tuli R, Huang X, Laquerriere P, Tuan RS, Li W-J, et al. Multilineage differentiation of human mesenchymal stem cells in a three-dimensional nanofibrous scaffold. Biomaterials. 2005;26(25):5158–5166
  2. Makino S, Fukuda K, Miyoshi S, Konishi F, Kodama H, Pan J, et al. Cardiomyocytes can be generated from marrow stromal cells in vitro. J Clin Invest. 1999;103(5):697–705
  3. Zeng L, Hu Q, Wang X, Mansoor A, Lee J, Feygin J, et al. Bioenergetic and functional consequences of bone marrow-derived multipotent progenitor cell transplantation in hearts with postinfarction left ventricular remodeling. Circulation. 2007;115(14):1866–1875
  4. Piao H, Youn TJ, Kwon JS, Kim YH, Bae JW, Bora S, et al. Effects of bone marrow derived mesenchymal stem cells transplantation in acutely infarcting myocardium. Eur J Heart Fail. 2005;7(5):730–738
  5. Orlic D, Kajstura J, Chimenti S, Jakoniuk I, Anderson SM, Li B, et al. Bone marrow cells regenerate infarcted myocardium. Nature. 2001;410(6829):701–705
  6. Hassink RJ, Brutel de la Riviere A, Mummery CL, Doevendans PA. Transplantation of cells for cardiac repair. J Am Coll Cardiol. 2003;41(5):711–717
  7. Bosnakovski D, Mizuno M, Kim G, Takagi S, Okumura M, Fujinaga T. Isolation and multilineage differentiation of bovine bone marrow mesenchymal stem cells. Cell Tissue Res. 2005;319(2):243–253
  8. Kawada H, Fujita J, Kinjo K, Matsuzaki Y, Tsuma M, Miyatake H, et al. Nonhematopoietic mesenchymal stem cells can be mobilized and differentiate into cardiomyocytes after myocardial infarction. Blood. 2004;104(12):3581–3587
  9. Pittenger MF, Mackay AM, Beck SC, Jaiswal RK, Douglas R, Mosca JD, et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143–147
  10. Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, et al. Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci. 2003;116(Pt 9):1827–1835
  11. Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell. 2007;131(5):861–872
  12. Rhodes NP, Srivastava JK, Smith RF, Longinotti C. Metabolic and histological analysis of mesenchymal stem cells grown in 3-D hyaluronan-based scaffolds. J Mater Sci Mater Med. 2004;15(4):391–395
  13. Fukuda K. Development of regenerative cardiomyocytes from mesenchymal stem cells for cardiovascular tissue engineering. Artif Organs. 2001;25(3):187–193
  14. Liu J, Hu Q, Wang Z, Xu C, Wang X, Gong G, et al. Autologous stem cell transplantation for myocardial repair. Am J Physiol Heart Circ Physiol. 2004;287(2):H501–H511
  15. Kovacic JC, Muller DW, Harvey R, Graham RM. Update on the use of stem cells for cardiac disease. Intern Med J. 2005;35(6):348–356
  16. Lyons AB, Parish CR. Determination of lymphocyte division by flow cytometry. J Immunol Methods. 1994;171(1):131–137
  17. Weber A, Pedrosa I, Kawamoto A, Himes N, Munasinghe J, Asahara T, et al. Magnetic resonance mapping of transplanted endothelial progenitor cells for therapeutic neovascularization in ischemic heart disease. Eur J Cardiothorac Surg. 2004;26(1):137–143
  18. Teng CJ, Luo J, Chiu RC, Shum-Tim D. Massive mechanical loss of microspheres with direct intramyocardial injection in the beating heart: implications for cellular cardiomyoplasty. J Thorac Cardiovasc Surg. 2006;132(3):628–632
  19. Dai W, Hale SL, Martin BJ, Kuang JQ, Dow JS, Wold LE, et al. Allogeneic mesenchymal stem cell transplantation in postinfarcted rat myocardium: short- and long-term effects. Circulation. 2005;112(2):214–223
  20. Choi D, Li D, Raisman G. Fluorescent retrograde neuronal tracers that label the rat facial nucleus: a comparison of Fast Blue, Fluoro-ruby, Fluoro-emerald, Fluoro-Gold and DiI. J Neurosci Methods. 2002;117(2):167–172
  21. Haugland R. Fluorescent lipophilic tracers. In: Handbook—a guide to fluorescent probes and labeling technologies. 10th ed. Molecular Probes™. Invitrogen 2005.14.4. p. 670–8.
  22. Povysil C, Dundr P, Tvrdik D, Podskubka A, Kana R, Horak M. Desmin-positive and alpha-smooth muscle actin positive chondrocytes in human defective articular cartilage—preliminary report. Cesk Patol. 2005;41(4):133–136

PII: S1443-9506(08)00010-3

doi: 10.1016/j.hlc.2008.01.006

Heart, Lung and Circulation
Volume 17, Issue 5 , Pages 395-403 , October 2008

Article Tools

* Image Usage & Resolution