Background:
Off-pump coronary artery bypass grafting is associated with transient periods of myocardial
ischemia during revascularization resulting in myocardial contractile dysfunction
and oxidative injury. The purpose of this study was to investigate the efficacy of
ethyl pyruvate as a myocardial protective agent in a rat model of off-pump coronary
artery bypass grafting associated with transient myocardial dysfunction without infarction.
Methods:
Wistar rats were subjected to transient ischemia via 10 min occlusion of the LAD coronary artery followed by 10 min of reperfusion. Animals received an IV bolus of Ringer's solution as a control
(n = 10) or Ringer's ethyl pyruvate (n = 10) immediately before the initiation of ischemia and reperfusion. Myocardial ATP
and lipid peroxidation levels were quantified for an estimation of energetics and
oxidative stress, respectively. In vivo cardiac function was assessed throughout the
ischemia and reperfusion periods.
Results:
Ethyl pyruvate significantly increased myocardial ATP levels compared to controls
(2650 ± 759 nmol/g versus 892 ± 276 nmol/g, p = 0.04). Myocardial oxidative stress was significantly reduced in animals treated with
ethyl pyruvate compared to controls (70.4 ± 2.6 nmol/g versus 81.8 ± 2.4 nmol/g, p = 0.04). dP/dt max and cardiac output were significantly greater in the ethyl pyruvate group compared
to controls during ischemia and reperfusion.
Conclusions:
Ethyl pyruvate enhances myocardial ATP levels, reduces oxidative stress, and preserves
myocardial function in a model of transient ischemia/reperfusion injury not subject
to myocardial infarction.
Keywords
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Article info
Footnotes
☆Presented at the 2003 American Heart Association Scientific Sessions, November 11, 2003.
Identification
Copyright
© 2005 Australasian Society of Cardiac and Thoracic Surgeons and the Cardiac Society of Australia and New Zealand. Published by Elsevier Inc. All rights reserved.
