Background
Our previous study has established a macaque model with early-phase endotoxic shock.
The present study further investigated myocardial and blood vessel injury in Macaques
by examining the subsequent expression of ACP, selectins, iNOS, and cTnI in response
to LPS treatment.
Methods
In an experiment with anaesthetised, instrumental macaques, eleven animals were randomised
into: an En group (n = 6), receiving a dose of 2.8 mg kg−1 lipopolysaccharides (LPS) by i.v.; and a Co group (n = 5), injected with normal saline of 1 ml kg−1. Cytochemistry of acid phosphatase (ACPase) in heart was performed by electron microscope
at 120 min following endotoxin injection. Three immunochemical stains, namely, L-selectin,
P-selectin and iNOS protein in heart, were studied. In addition, cardiac troponin
I (cTnI), L-selectin and P-selectin in plasma were detected.
Results
In the early phase of endotoxic shock, LPS caused myocardial lysosome damage. The
data of immunochemical staining showed the thrombus formation in vessels and the increase
of iNOS, L-Selectin and P-Selectin expression in heart, but LPS challenge did not
change L-selectin, P-selectin and cTnI in plasma.
Conclusion
The increase of iNOS, L-selectin and P-selectin protein expression following endotoxin
administration may have caused vessel injury and myocardial damage in macaques.
Keywords
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Article info
Publication history
Accepted:
October 11,
2006
Received in revised form:
October 6,
2006
Received:
July 10,
2006
Identification
Copyright
© 2006 Australasian Society of Cardiac and Thoracic Surgeons and the Cardiac Society of Australia and New Zealand. Published by Elsevier Inc. All rights reserved.
