Background
The noble gas anaesthetic, xenon has previously been shown to protect the adult myocardium
from ischaemia/reperfusion (I/R) injury, however its effect on immature myocardium
is unclear. The aim of this study was to investigate the effect of xenon on the isolated
immature heart.
Methods
Isolated, immature (2–3 weeks old) New Zealand rabbit hearts were perfused with Krebs–Henseleit buffer via
Langendorff-mode. After 20 min of baseline equilibration, hearts were pretreated with 75% xenon, 75% xenon + 100 μM diazoxide, or 75% xenon + 100 μM 5-hydroxydecanoate, and then subjected to 1 h of global ischaemia and 3 h of reperfusion.
Results
Pretreatment with 75% xenon significantly improved cardiac function (P < 0.01 vs. the I/R group, respectively), limited myocardial infarct size (20.83 ± 2.16%, P < 0.01 vs. 35.82 ± 2.14% of the I/R group), reduced cardiac enzyme release (CK-MB, 1.00 ± 0.19 IU/L, P < 0.01 vs. 0.44 ± 0.14 IU/L of the I/R group; LDH, 6.15 ± 1.06 IU/L P < 0.01 vs. 3.49 ± 0.37 IU/L of the I/R group) and decreased apoptosis (6.17 ± 0.56%, P < 0.01 vs. 11.31 ± 0.93% of the I/R group). In addition, the mitochondrial structure changes caused by
I/R injury were largely prevented by 75% xenon pretreatment (1.37 ± 0.16, P < 0.01 vs. 2.32 ± 0.13 of the I/R group). The mitochondrial adenosine triphosphate-sensitive potassium
(mitoKATP) channel opener diazoxide did not influence the effect of xenon, but the specific
mitoKATP channel blocker 5-hydroxydecanoate completely abolished this effect.
Conclusions
Our study demonstrated that pretreatment with 75% xenon protected immature heart from
I/R injury, and this protection was probably mediated by preservation of myocardial
mitochondria and opening of mitoKATP channel.
Keywords
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Article info
Publication history
Published online: December 26, 2012
Accepted:
October 30,
2012
Received in revised form:
October 29,
2012
Received:
September 13,
2012
Identification
Copyright
© 2012 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ). Published by Elsevier Inc. All rights reserved.