Background
Infusion with hydrogen gas-saturated saline has recently been reported to exert antioxidant
and anti-inflammatory activity that may protect against organ damage induced by oxidative
stress. Therefore because oxidative stress plays a significant role in the pathophysiology
of myocardial infarction (MI), the aim of our study was to investigate whether hydrogen-rich
saline has cardioprotective effects against isoproterenol-induced MI in rats.
Methods
An acute MI model was induced in male Wistar rats by subcutaneous injection of isoproterenol.
Different doses of hydrogen-rich saline (5, 7.5, and 10 mL/kg body weight i.p.) or Vitamin C (250 mg/kg body weight i.g.) were administered to the rats. Oxidative stress indices including
levels of myocardial marker enzymes, inflammatory cytokines, membrane-bound myocardial
enzymes and histopathological changes were measured.
Results
Compared with those in isoproterenol-MI group, hydrogen-rich saline decreased malondialdehyde
and 8-hydroxy-desoxyguanosine concentrations, enhanced superoxide dismutase and Na+-K+-ATPase activity, lowered Ca2+-ATPase activity and decreased interleukin-6 and tumour necrosis factor-α levels in
the serum and/or cardiac tissue of rats. Hydrogen-rich saline pretreatment also diminished
infarct size, improved left heart function, and ameliorated pathological changes of
the left heart.
Conclusion
From these results, hydrogen-rich saline exerts cardiovascular protective effects
against isoproterenol-induced MI at least in part via interactions which evoke antioxidant
and anti-inflammatory activities.
Keywords
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Article info
Publication history
Published online: December 04, 2014
Accepted:
November 24,
2014
Received in revised form:
November 18,
2014
Received:
July 30,
2014
Identification
Copyright
© 2014 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ). Published by Elsevier Inc. All rights reserved.
